Immunolocalization indicates plasmodesmal trafficking of storage proteins during cambial reactivation in Populus nigra.
Identifieur interne : 003221 ( Main/Exploration ); précédent : 003220; suivant : 003222Immunolocalization indicates plasmodesmal trafficking of storage proteins during cambial reactivation in Populus nigra.
Auteurs : Maike Fuchs [Allemagne] ; Katrin Ehlers ; Torsten Will ; Aart J E. Van BelSource :
- Annals of botany [ 1095-8290 ] ; 2010.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Seed Storage Proteins.
- chemical : Fluorescent Dyes.
- physiology : Populus.
- Blotting, Western, Electrophoresis, Polyacrylamide Gel, Immunohistochemistry, Microscopy, Electron, Transmission, Protein Transport.
Abstract
BACKGROUND AND AIMS
Cambium reactivation after dormancy and budbreak in deciduous trees requires a supply of mobilized reserve materials. The pathway and mode of transfer of these materials are poorly understood.
METHODS
Transport of reserve materials during cambium reactivation in Populus nigra was investigated by conventional and immunocytochemical TEM analyses, SDS-PAGE, western blotting and intracellular microinjection of fluorescent dyes.
KEY RESULTS
Proteinaceous compounds stored in vacuoles and protein bodies of vascular cells and ray cells disappeared within 3 weeks after cambial reactivation and budbreak. Some of these proteins (32 kDa, 30 kDa and 15 kDa) were labelled by lectin antibodies in SDS-PAGE. The same antibodies were localized to plasmodesmata (PDs) between phloem parenchyma, ray cells and fusiform cambial cells. In addition, proteinaceous particles were localized inside the cytoplasmic sleeves of these PDs during budbreak. During this period, the functional diameter of PDs was about 2.2 nm which corresponds approximately to the Stokes' radius of the detected 15-kDa protein.
CONCLUSIONS
Lectin-like reserve proteins or their degradation products seem to be transferred through PDs of phloem parenchyma and rays during cambial reactivation and budbreak. PD transfer of storage proteins is a novelty which supports the concept of symplasmic nutrient supply to the cambial region.
DOI: 10.1093/aob/mcq130
PubMed: 20584737
PubMed Central: PMC2924828
Affiliations:
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Van Bel</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20584737</idno><idno type="pmid">20584737</idno><idno type="doi">10.1093/aob/mcq130</idno><idno type="pmc">PMC2924828</idno><idno type="wicri:Area/Main/Corpus">003141</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003141</idno><idno type="wicri:Area/Main/Curation">003141</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003141</idno><idno type="wicri:Area/Main/Exploration">003141</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Immunolocalization indicates plasmodesmal trafficking of storage proteins during cambial reactivation in Populus nigra.</title><author><name sortKey="Fuchs, Maike" sort="Fuchs, Maike" uniqKey="Fuchs M" first="Maike" last="Fuchs">Maike Fuchs</name><affiliation wicri:level="1"><nlm:affiliation>Institute of General Botany, Justus-Liebig-University, Senckenbergstrasse 17, Giessen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of General Botany, Justus-Liebig-University, Senckenbergstrasse 17, Giessen</wicri:regionArea><wicri:noRegion>Giessen</wicri:noRegion><wicri:noRegion>Giessen</wicri:noRegion><wicri:noRegion>Giessen</wicri:noRegion></affiliation></author><author><name sortKey="Ehlers, Katrin" sort="Ehlers, Katrin" uniqKey="Ehlers K" first="Katrin" last="Ehlers">Katrin Ehlers</name></author><author><name sortKey="Will, Torsten" sort="Will, Torsten" uniqKey="Will T" first="Torsten" last="Will">Torsten Will</name></author><author><name sortKey="Van Bel, Aart J E" sort="Van Bel, Aart J E" uniqKey="Van Bel A" first="Aart J E" last="Van Bel">Aart J E. Van Bel</name></author></analytic><series><title level="j">Annals of botany</title><idno type="eISSN">1095-8290</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Blotting, Western (MeSH)</term><term>Electrophoresis, Polyacrylamide Gel (MeSH)</term><term>Fluorescent Dyes (MeSH)</term><term>Immunohistochemistry (MeSH)</term><term>Microscopy, Electron, Transmission (MeSH)</term><term>Populus (physiology)</term><term>Protein Transport (MeSH)</term><term>Seed Storage Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Colorants fluorescents (MeSH)</term><term>Immunohistochimie (MeSH)</term><term>Microscopie électronique à transmission (MeSH)</term><term>Populus (physiologie)</term><term>Protéines de stockage des graines (métabolisme)</term><term>Technique de Western (MeSH)</term><term>Transport des protéines (MeSH)</term><term>Électrophorèse sur gel de polyacrylamide (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Seed Storage Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Fluorescent Dyes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines de stockage des graines</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Western</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Immunohistochemistry</term><term>Microscopy, Electron, Transmission</term><term>Protein Transport</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Colorants fluorescents</term><term>Immunohistochimie</term><term>Microscopie électronique à transmission</term><term>Technique de Western</term><term>Transport des protéines</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND AND AIMS</b></p><p>Cambium reactivation after dormancy and budbreak in deciduous trees requires a supply of mobilized reserve materials. The pathway and mode of transfer of these materials are poorly understood.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS</b></p><p>Transport of reserve materials during cambium reactivation in Populus nigra was investigated by conventional and immunocytochemical TEM analyses, SDS-PAGE, western blotting and intracellular microinjection of fluorescent dyes.</p></div><div type="abstract" xml:lang="en"><p><b>KEY RESULTS</b></p><p>Proteinaceous compounds stored in vacuoles and protein bodies of vascular cells and ray cells disappeared within 3 weeks after cambial reactivation and budbreak. Some of these proteins (32 kDa, 30 kDa and 15 kDa) were labelled by lectin antibodies in SDS-PAGE. The same antibodies were localized to plasmodesmata (PDs) between phloem parenchyma, ray cells and fusiform cambial cells. In addition, proteinaceous particles were localized inside the cytoplasmic sleeves of these PDs during budbreak. During this period, the functional diameter of PDs was about 2.2 nm which corresponds approximately to the Stokes' radius of the detected 15-kDa protein.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>Lectin-like reserve proteins or their degradation products seem to be transferred through PDs of phloem parenchyma and rays during cambial reactivation and budbreak. PD transfer of storage proteins is a novelty which supports the concept of symplasmic nutrient supply to the cambial region.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20584737</PMID><DateCompleted><Year>2010</Year><Month>12</Month><Day>02</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1095-8290</ISSN><JournalIssue CitedMedium="Internet"><Volume>106</Volume><Issue>3</Issue><PubDate><Year>2010</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Annals of botany</Title><ISOAbbreviation>Ann Bot</ISOAbbreviation></Journal><ArticleTitle>Immunolocalization indicates plasmodesmal trafficking of storage proteins during cambial reactivation in Populus nigra.</ArticleTitle><Pagination><MedlinePgn>385-94</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/aob/mcq130</ELocationID><Abstract><AbstractText Label="BACKGROUND AND AIMS" NlmCategory="OBJECTIVE">Cambium reactivation after dormancy and budbreak in deciduous trees requires a supply of mobilized reserve materials. The pathway and mode of transfer of these materials are poorly understood.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">Transport of reserve materials during cambium reactivation in Populus nigra was investigated by conventional and immunocytochemical TEM analyses, SDS-PAGE, western blotting and intracellular microinjection of fluorescent dyes.</AbstractText><AbstractText Label="KEY RESULTS" NlmCategory="RESULTS">Proteinaceous compounds stored in vacuoles and protein bodies of vascular cells and ray cells disappeared within 3 weeks after cambial reactivation and budbreak. Some of these proteins (32 kDa, 30 kDa and 15 kDa) were labelled by lectin antibodies in SDS-PAGE. The same antibodies were localized to plasmodesmata (PDs) between phloem parenchyma, ray cells and fusiform cambial cells. In addition, proteinaceous particles were localized inside the cytoplasmic sleeves of these PDs during budbreak. During this period, the functional diameter of PDs was about 2.2 nm which corresponds approximately to the Stokes' radius of the detected 15-kDa protein.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">Lectin-like reserve proteins or their degradation products seem to be transferred through PDs of phloem parenchyma and rays during cambial reactivation and budbreak. PD transfer of storage proteins is a novelty which supports the concept of symplasmic nutrient supply to the cambial region.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Fuchs</LastName><ForeName>Maike</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute of General Botany, Justus-Liebig-University, Senckenbergstrasse 17, Giessen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ehlers</LastName><ForeName>Katrin</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Will</LastName><ForeName>Torsten</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>van Bel</LastName><ForeName>Aart J E</ForeName><Initials>AJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>06</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Ann Bot</MedlineTA><NlmUniqueID>0372347</NlmUniqueID><ISSNLinking>0305-7364</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005456">Fluorescent Dyes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D055314">Seed Storage Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005456" MajorTopicYN="N">Fluorescent Dyes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007150" 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Katrin" sort="Ehlers, Katrin" uniqKey="Ehlers K" first="Katrin" last="Ehlers">Katrin Ehlers</name><name sortKey="Van Bel, Aart J E" sort="Van Bel, Aart J E" uniqKey="Van Bel A" first="Aart J E" last="Van Bel">Aart J E. Van Bel</name><name sortKey="Will, Torsten" sort="Will, Torsten" uniqKey="Will T" first="Torsten" last="Will">Torsten Will</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Fuchs, Maike" sort="Fuchs, Maike" uniqKey="Fuchs M" first="Maike" last="Fuchs">Maike Fuchs</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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